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Introduction: Occupational exposure to ionizing radiation can potentially lead to adverse health effects, including cancer and genetic defects. Genetic damage caused by radiation can be detected if micronuclei are observed. The objective of this pilot study was to detect the presence of micronuclei in cells of the oral mucosa in inidividuals occupationally exposed to ionizing radiation.
Methods: We implemented a pilot case-control study in which we compared oral mucosa micronuclei in 30 medical and nursing personnel in radiology centers in Celaya, Mexico, with 30 volunteers not exposed to ionizing radiation recruited from a public University. The oral mucosa was brushed and the amount of micronuclei was quantified. Chi-square test or t-test for two proportions were used to compared ionizing radiation and genetic damage between exposed and non-exposed groups.
Results: The exposed group had an average of 5.37 ± 3.49 micronuclei and the non-exposed had 0.37 ± 0.61 (P<0.01). In the exposed group, 90% of participants exhibited genetic damage compared to 6.67% in the unexposed group (P<0.05).
Conclusion: In this pilot study, medical and nursing staff from radiology centers presented with higher genetic damage compared to control group. Further studies are needed to identify the prevalence of genetic damage due to occupational radiation exposure in Mexico.
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Revised 7/16/2018. Revision Description: Removed outdated link.
World Health Organization. Ionizing radiation, health effects and and protective measures. Fact sheets N° 371. World Health Organization 2018. http://www.who.int/news-room/fact-sheets/detail/ionizing-radiation-health-effects-and-protective-measures Accessed 3 May, 2019.
Caicedo R, Argüelles G, Alzate AJCM. Exposición a dosis bajas de radiación ionizante en el Hospital Universitario del Valle, Cali, 1980-1992. 1996;27(3, 4):134-137.
International Commission on Radiological Protection. Dose Limits. ICROAEDIA. http://www.icrp.org/icrpaedia/limits.asp Accessed 3 May, 2019.
Albertini RJ, Anderson D, Douglas GR, et al. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutation research. 2000;463(2):111-172.
Zalacain M, Sierrasesumaga L, Patino A. [The cytogenetic assay as a measure of genetic instability induced by genotoxic agents]. Anales del sistema sanitario de Navarra. 2005;28(2):227-236.
Norppa H, Falck GC-MJM. What do human micronuclei contain? 2003;18(3):221-233.
Fenech MJMRF, Mutagenesis MMo. The in vitro micronucleus technique. 2000;455(1-2):81-95.
Lindberg HK, Wang X, Järventaus H, et al. Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes. 2007;617(1-2):33-45.
Popova L, Kishkilova D, Hadjidekova V, et al. Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography. 2007;36(3):168-171.
da Silva AE, Rados PV, da Silva Lauxen I, et al. Nuclear changes in tongue epithelial cells following panoramic radiography. 2007;632(1-2):121-125.
Holland N, Bolognesi C, Kirsch-Volders M, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. 2008;659(1-2):93-108.
Norma Oficial Mexicana -SSA1-2002, Salud ambiental. Requisitos técnicos para las instalaciones, responsabilidades sanitarias, especificaciones técnicas para los equipos y protección radiológica en establecimientos de diagnóstico médico con rayos X (12)), Diario Oficial, 2006. http://www.cenetec.salud.gob.mx/descargas/equipoMedico/normas/NOM_229_SSA1_2002.pdf. Accessed May 3, 2019.
Stranden E, Widmark A, Sekse TJAR. Assessing doses to interventional radiologists using a personal dosimeter worn over a protective apron. 2008;49(4):415-418.
Nassef M, Kinsara AJJoTUfS. Occupational radiation dose for medical workers at a university hospital. 2017;11(6):1259-1266.
Thomas P, Holland N, Bolognesi C, et al. Buccal micronucleus cytome assay. 2009;4(6):825.
Qian Q-Z, Cao X-K, Shen F-H, Wang QJRpd. Effects of ionising radiation on micronucleus formation and chromosomal aberrations in Chinese radiation workers. 2015;168(2):197-203.
Kanagaraj K, Basheerudeen SAS, Jose M, et al. Assessment of dose and DNA damages in individuals exposed to low dose and low dose rate ionizing radiations during computed tomography imaging. 2015;789:1-6.