Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics

Main Article Content

Lyudmila Trenozhnikova
Azliyati Azizan


Introduction: Antimicrobial-resistant pathogens pose serious challenges to healthcare institutions and health of the public. Thus, there is an urgent need for the discovery of new and effective antimicrobial agents.  Microorganisms that exist in extreme environments such as those with high salinity or alkalinity, are known as extremophiles, and include various species of actinomycetes. The goal of this study is to discover novel antibiotics from extremophiles found in Kazakhstan that are effective against drug resistant pathogens.

Methods: Soil from extreme environments of Kazakhstan was collected, and pure cultures of actinomycetes were isolated and cultured in modified Bennett’s broth with either high concentrations of salt or high pH to mimic extreme environments. Extracts obtained from selected actinomycetes strains were used to test for antimicrobial activity against Staphylococcus aureus, Escherichia coli and Aspergillus niger.

Results: A total of 5936 strains of extremophile actinomycetes were isolated; from these, 2019 strains were further isolated into pure cultures. Of these, 415 actinomycetes strains that demonstrated antagonistic antibacterial activities were selected.  These actinomycetes were further classified into groups and subgroups based on their responses to different culture conditions. Antimicrobial antagonism activity for some of the actinomycetes strains was dependent on culture conditions and development of  aerial mycelia under extreme conditions.

Conclusion: We identified several interesting candidate extracts with putative antimicrobial activities against several strains of drug resistant pathogens. Our research of the actinomycetes’ ability to produce antibiotics in the near-natural conditions provides a great opportunity to assess their biodiversity and distribution in the Central Asian region and to develop new methodological approaches to the screening of new antimicrobial agents.

Article Details

How to Cite
Trenozhnikova, L., & Azizan, A. (2018). Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics. Central Asian Journal of Global Health, 7(1).
Author Biography

Azliyati Azizan, Nazarbayev University School of Medicine, Astana, Kazakhstan

Associate Professor

Biomedical Sciences Department


Singer, R.S., R. Finch, H.C. Wegener, R. Bywater, J. Walters and M. Lipsitch, 2003. Antibiotic resistance – the interplay between antibiotic use in animals and human beigs. The Lancet Infect. Dis., 3: 47-51.

Talbot, G.H., J. Bradley, Jr. J.E. Edwards, D. Gilbert, M. Scheld and J.G. Bartlett, 2006. Bad bags Need Drugs: An update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America. Clin. Infect. Dis., 42: 657-668.

Newman, D.J. and M.G. Cragg, 2007. Natural products as sources of new drugs over the last 25 years. J. Nat. Prod., 70: 461-477.

Keiser, T., M.J. Bibb, M.J. Buttner, K.F. Chater and D.A. Hopwood, 2000. General introduction to actinomycete biology. Proceeding of the Practical Streptomyces Genetics, The John Innes Foundation, Croves, Norwich, England, pp: 1-21.

Demain A.L., 1999. Pharmaceutically active secondary metabolites of microorganisms. Appl. Microbiol. Biotechnol., 52: 455–63.

Basilio A., I. Gonzalez, M.F. Vicente, J. Gorrochategui, A. Cabello, A. Gonzalez, 2003. Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. J. Appl. Microbiol., 95: 814-23.

Zhang, A. and A.L. Demain, 2005. Natural Products. Drug Discovery and Therapeutical Medicine. 382p.

Feling, R., G. Buchanan, T. Mincer, C. Kauffman, P. Jensen, W. Fenical, 2003. Salinisporamide A: A highly cytotoxic proteasome inhibitor from a novel microbial source, a marine bacterium of the new genus Salinispora. Angew. Chem. Int. Ed. 42: 355-357.

Rainey А. and A. Oren, 2006. Extremophiles, 35. Academic Press. 838p.

Debananda S. Ningthoujam, Pintubala Kshetri, Suchitra Sanasam and Salam Nimaichand, 2009. Screening, Identification of Best Producers and Optimization of Extracellular Proteases from Moderately Halophilic Alkalithermotolerant Indigenous Actinomycetes. World Applied. Sciences Journal 7 (7): 907-916.

K. Suthindhiran and K. Kannabiran, 2009. Cytotoxic and Antimicrobial Potential Actinomycete Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India. American Journal of Infectious Diseases 5 (2): 90-98.

Vasavada, S. H., J. T.Thumar and S. P. Singh, 2006. Secretion of a potent antibiotic by salt-tolerant and alkaliphilic actinomycete Streptomyces sannanensis strain RJT-1. Current Science. 91(10):1393-1397.

Dietera, A., A. Hamm, H. P. Fiedler, M. Goodfellow, W. E. Muller, R. Brun and G. Bringmann, 2003. Pyrocoll, an antibiotic, antiparasitic and antitumor compound produced by a novel alkaliphilic Streptomyces strain. J. Antibiot. 56: 639–646.

Kokare, C. R., K. R.Mahadik, S. S. Kadam and B. A. Chopade, 2004. Isolation, characterization and antimicrobial activity of marine halophilic Actinopolyspora species AH1 from the west coast of India. Curr. Sci. 86: 593–597

Manam, R. R. et al., 2005. Lajollamycin, a nitro-tetraenespiro-beta-lactone-gamma-lactum antibiotic from the marine actinomycetes Streptomyces nodosus. J. Nat. Prod. 68: 240–243.

Fiedler, H. P. et al., 2005. Marine actinomycetes as a source of novel secondary metabolites. Antonie Van Leeuwenhoek. 87: 37–42.

Jensen, P., P. Williams, D-C. Oh, L. Zeigler, W. Fenical, 2007. Species-specific secondary metabolite production in marine actinomycetes of the genus Salinispora. J. Appl. and Environ. Microbiol. 73: 1146-1152.

Phoebe, C.H., J. Cambie, F.G. Albert, K. Van Tran, J. Cabrera, H.J. Correira, Y. Guo, J. Lindermuth, et al., 2001. Extremophilic organisms as an unexplored source of antifungal compounds. Journal of Antibiotics 54: 56–65.

Demain, A. L. 1992. Microbial secondary metabolism: a new theoretical frontier for academia, a new opportunity for industry. CIBA Found. Symp. 171:3-16.

Champness, W.C., Chater K.F., 1994. Regulation and integration of antibiotic production and morphological differentiation in Streptomyces spp. In: Piggot PJ, Morgan CP, Youngman P (eds) Regulation of bacterial development. American Society for Microbiology Press, Washington, DC, pp 61–93.

Demain, A., 1995. Why do microorganisms produce antimicrobials? Proceeding of the Symposium on Society of General Microbiology, 1995, Cambridge University Press, Cambridge, pp: 205-228.

Demain, A. L., and A. Fang. 1995. Emerging concepts of secondary metabolism in actinomycetes. Actinomycetologica 9: 98-117.

Chater KF, Bibb MJ (1997) Regulation of bacterial antibiotic production. In: Kleinkauf H, von Döhren H (eds) Biotechnology, products of secondary metabolism. VCH, Weinheim, pp 59–105.

Bibb, M.J., 2005. Regulation of secondary metabolism in Streptomycetes. Curr. Opin. Microbiol., 8: 208-215.

Gesheva V. and R. Gesheva, 1993. Structure of the population of Streptomyces hygroscopicus and characteristics of its variants. Actinomycetes. 4 (3): 65-71.